MersV1, Main, Exploration, bibRecord, 002C90

Single-based resolution for oligodeoxynucleotides and their phosphorothioate modifications by replaceable capillary gel electrophoresis.

Identifieur interne : 002C90 ( Main/Exploration ); précédent : 002C89; suivant : 002C91

Single-based resolution for oligodeoxynucleotides and their phosphorothioate modifications by replaceable capillary gel electrophoresis.

Auteurs : Rong Chen [République populaire de Chine] ; Xuefang Luo ; Xin Di ; Ying Li ; Yuqing Sun ; Yuzhu Hu

Source :

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [ 1570-0232 ] ; 2006.

RBID : pubmed:16809077

Descripteurs français

KwdFr :
- Acétonitriles, Conformation d'acide nucléique, Oligodésoxyribonucléotides (isolement et purification), Organothiophosphates (), Organothiophosphates (isolement et purification), Polyéthylène glycols, Urée, Électrophorèse capillaire (), Électrophorèse capillaire (instrumentation).
MESH :
- isolement et purification : Oligodésoxyribonucléotides, Organothiophosphates, Électrophorèse capillaire.
- Acétonitriles, Conformation d'acide nucléique, Organothiophosphates, Polyéthylène glycols, Urée, Électrophorèse capillaire.

English descriptors

KwdEn :
- Acetonitriles, Electrophoresis, Capillary (instrumentation), Electrophoresis, Capillary (methods), Nucleic Acid Conformation, Oligodeoxyribonucleotides (isolation & purification), Organothiophosphates (chemistry), Organothiophosphates (isolation & purification), Polyethylene Glycols, Urea.
MESH :
- chemical , chemistry : Organothiophosphates.
- chemical , isolation & purification : Oligodeoxyribonucleotides, Organothiophosphates.
- chemical : Acetonitriles, Polyethylene Glycols, Urea.
- instrumentation : Electrophoresis, Capillary.
- methods : Electrophoresis, Capillary.
- Nucleic Acid Conformation.

Abstract

A replaceable capillary gel electrophoretic (replaceable CGE) method was developed for the separation of two sets of model compounds of single-stranded oligodeoxynucleotide mixtures (18-20 mers), phosphodiester oligodeoxynucleotides (PO-ODNs) and their phosphorothioate modifications (PS-ODNs), with equal sequences differing in a single base. Polyethylene glycol (PEG) 35000 was chosen as the sieving matrix. It was confirmed that PEG polymer solution less influenced resolutions of the PS-ODNs compared with those of the PO-ODNs, while acetonitrile used as an additive in the system improved the separation significantly. It was also noticed that the effect of temperature on separation was much larger than that of denaturant urea.

DOI: 10.1016/j.jchromb.2006.05.034
PubMed: 16809077

Affiliations:

République populaire de Chine

Le document en format XML

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Serveur d'exploration MERS

Single-based resolution for oligodeoxynucleotides and their phosphorothioate modifications by replaceable capillary gel electrophoresis.

Single-based resolution for oligodeoxynucleotides and their phosphorothioate modifications by replaceable capillary gel electrophoresis.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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